Utilization of an In Silico 16S Ribosomal RNA Gene-Based Restriction Enzyme Approach for Identification of Lactic Acid Bacteria

In silico 16S ribosomal RNA gene-based restriction enzyme approach is a computer-simulated polymerase chain reaction-restriction fragment length polymorphism analysis method, namely customizable in sequence evaluation for sites. The purpose of this study was to identify lactic acid bacteria using gene (16S polymorphism). An investigation conducted detect species-specific molecular markers different strains. We selected six out 20 sequences from probiotic-potential Initially, the corresponding were database aligned Clustal Omega program and their evolutionary relationships determined. results indicated that studied strains belonged 6 distinct clades except genus Lactobacillus. Furthermore, reactionrestriction further taxonomic classification. Different enzymes used recognize sites present on genes. Customizable output comprised predicted agarose gel as virtual patterns, proper selection appropriate (s) be marker generation. Our demonstrated ApyPI, CchIII NheI produced patterns those Ku324937_C species. most abundant seen Ku324928_C (10 markers), followed by Ku324908_C (8 markers) order Hemiptera, then Ku324909_C (5 Ku324898_C (4 finally, (3 markers). powerful tool provide better understanding organisms found raw and/or fermented milk.